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Commit d023d5d9 authored by Laura Cook's avatar Laura Cook
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add section on library complexity ENCODE guidelines

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......@@ -339,6 +339,17 @@ Total estimated number of unique 150-mers: 2740338543
### rule get_picard_complexity_metrics:
Library Complexity
ChIP-seq Standards:
| PBC1 | PBC2 | Bottlenecking level | NRF | Complexity | Flag colors |
|:-:|:-:|:-:|:-:|:-:|:-:|
| < 0.5 | < 1 | Severe | < 0.5 | Concerning | Orange |
| 0.5 ≤ PBC1 < 0.8 | 1 ≤ PBC2 < 3 | Moderate | 0.5 ≤ NRF < 0.8 | Acceptable | Yellow |
| 0.8 ≤ PBC1 < 0.9 | 3 ≤ PBC2 < 10 | Mild | 0.8 ≤ NRF < 0.9 | Compliant | None |
| ≥ 0.9 | ≥ 10 | None | > 0.9 | Ideal | None |
# 5. deepTools
### rule deeptools_summary:
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