diff --git a/cross_species_comparison/README.md b/cross_species_comparison/README.md
index 31d70ab34236bba9e4e2b2676dab245ee8f69a99..1b63dab593aff5e97b3219a7459f0d5faf2af3fe 100644
--- a/cross_species_comparison/README.md
+++ b/cross_species_comparison/README.md
@@ -214,7 +214,6 @@ chainCleaner improves the specificity in genome alignment chains by detecting an
 
 ```
 chainCleaner smiCra1_mm10_repFil.chain mm10.2bit smiCra1.2bit smiCra1_mm10_repFill_chainCl.chain smiCra1_mm10_repFill_chainCl.bed -tSizes=mm10.chrom.sizes -qSizes=smiCra1.chrom.sizes -LRfoldThreshold=2.5 -doPairs -LRfoldThresholdPairs=10 -maxPairDistance=10000 -maxSuspectScore=100000 -minBrokenChainScore=75000 -linearGap=loose
-
 ```
 
 `-LRfoldThreshold` = threshold for removing local alignment blocks if the score of the left and right fill of brokenChain. Default is 2.5