diff --git a/dunnart/configs/.multiqc_config.yaml b/dunnart/configs/.multiqc_config.yaml index d6ec7612b1ea109d778c6c15e34a2d3cc0084a74..2a5e8c7d1c356b4dae82866f29674a8324ec0a3d 100644 --- a/dunnart/configs/.multiqc_config.yaml +++ b/dunnart/configs/.multiqc_config.yaml @@ -2,6 +2,15 @@ title: "Histone ChIP-seq QC for dunnart craniofacial tissue: H3K4me3 & H3K27ac" intro_text: "" report_comment: "Generated on DATE" +run_modules: + - custom_content + - fastqc + - samtools + - picard + - preseq + - deeptools + - phantompeakqualtools + report_header_info: - Contact E-mail: 'lecook@student.unimelb.edu.au' @@ -10,12 +19,12 @@ report_header_info: module_order: - MACS2 - MACS2_narrowPeak_count - - deepTools - - Preseq: + - deeptools + - preseq: genome_size: 3074798085 read_length: 150 - - Samtools - - Picard + - samtools + - picard - phantompeakqualtools - bowtie2: name: 'Bowtie2' @@ -24,33 +33,84 @@ module_order: log_filesize_limit: 5000000000 -custom_data: - encode_metrics: - plot_type: 'generalstats' - file_format: 'tsv' - pconfig: - - NRF: - namespace: "NRF" - title: "NRF" - description: "ratio between the number of positions in the genome that uniquely mapped reads map to and the total number of uniquely mappable reads" - max: 1 - min: 0 - scale: 'RdYlGn' - - PBC1: - namespace: "PBC1" - title: "PBC1" - description: "ratio of genomic locations with EXACTLY one read pair over the genomic locations with AT LEAST one read pair" - max: 1 - min: 0 - scale: 'RdYlGn' - - PBC2: - namespace: "PBC2" - title: "PBC2" - description: "ratio of genomic locations with EXACTLY one read pair over the genomic locations with EXACTLY two read pairs" - max: 10 - min: 0 - scale: 'RdYlGn' +id: 'frip_score' +section_name: 'MACS2 FRiP score' +plot_type: 'bargraph' +anchor: 'frip_score' +pconfig: + title: 'FRiP score' + ylab: 'FRiP score' + ymax: 1 + ymin: 0 + tt_decimals: 2 + +id: 'peak_count' +section_name: 'MACS2 peak count' +description: "is calculated from total number of peaks called by + <a href='https://github.com/taoliu/MACS' target='_blank'>MACS2</a>" +plot_type: 'bargraph' +anchor: 'peak_count' +pconfig: + title: 'Total peak count' + ylab: 'Peak count' + +id: 'strand_shift_correlation' +section_name: 'spp strand-shift correlation' +description: "generated using run_spp.R script from + <a href='https://github.com/kundajelab/phantompeakqualtools' target='_blank'>phantompeakqualtools</a>." +plot_type: 'linegraph' +anchor: 'strand_shift_correlation' +pconfig: + title: 'Strand-shift correlation plot' + ylab: 'Cross-correlation' + xlab: 'Strand-shift (bp)' + xDecimals: False + tt_label: 'Strand-shift (bp) {point.x}: {point.y:.2f} Cross-correlation' + +id: 'nsc_coefficient' +section_name: 'spp NSC coefficient' +description: "generated using run_spp.R script from + <a href='https://github.com/kundajelab/phantompeakqualtools' target='_blank'>phantompeakqualtools</a>." +plot_type: 'bargraph' +anchor: 'nsc_coefficient' +pconfig: + title: 'Normalized strand cross-correlation coefficient' + ylab: 'NSC coefficient' + ymin: 1 + tt_decimals: 1 + +id: 'rsc_coefficient' +section_name: 'spp RSC coefficient' +description: "generated using run_spp.R script from + <a href='https://github.com/kundajelab/phantompeakqualtools' target='_blank'>phantompeakqualtools</a>." +plot_type: 'bargraph' +anchor: 'rsc_coefficient' +pconfig: + title: 'Relative strand cross-correlation coefficient' + ylab: 'RSC coefficient' + ymin: 0 + tt_decimals: 1 + + +report_section_order: + frip_score: + before: peak_count + strand_shift_correlation: + before: peak_annotation + nsc_coefficient: + before: strand_shift_correlation + rsc_coefficient: + before: nsc_coefficient + +custom_plot_config: + picard_insert_size: + cpswitch_c_active: False + smooth_points: 1000 sp: - encode_metrics: - fn: '*encodeQC.txt' + preseq: + fn: '*.ccurve.txt' + deeptools/plotFingerprintOutRawCounts: + fn: '*plotFingerprint*' + deeptools/plotProfile: + fn: '*plotProfile*' diff --git a/dunnart/configs/SRR.txt b/dunnart/configs/SRR.txt index ed29c6a9951ab62356143dab6d195308ee5e35b7..0e334a7318975dfaaed453c26ab3ada7d463e38d 100644 --- a/dunnart/configs/SRR.txt +++ b/dunnart/configs/SRR.txt @@ -1,5 +1,5 @@ -IP Input -A-2_H3K4me3 A-1_input -A-3_H3K27ac A-1_input -B-2_H3K4me3 B-1_input -B-3_H3K27ac B-1_input +IP Input mark +A-2_H3K4me3 A-1_input H3K4me3 +A-3_H3K27ac A-1_input H3K27ac +B-2_H3K4me3 B-1_input H3K4me3 +B-3_H3K27ac B-1_input H3K27ac